Veteran dog trainer and physical comedian J.R. Johns and his rescued partners bring to the stage a wild romp through everything that is humorous and endearing about dogs. J.R. has the ability to allow his canine partners to actually develop as characters then interact with them as the continuous brunt of their pranks on stage. 
J.R. Johns & His Best Friends's audition in Episode 101 consisted of J.R.'s dogs performing handstands, walking on their hind legs, and hopping over J.R. Piers Morgan, Brandy Norwood, and David Hasselhoff all voted "Yes", sending the act to the Semifinals.
J.R. Johns & His Best Friends' Semifinals performance in Episode 104 consisted of performing magic tricks with his dogs such as creating the illusion of dogs changing clothes behind a curtain. Piers Morgan, Brandy Norwood, and David Hasselhoff all gave J.R. "X" votes. J.R. did not receive enough votes to finish in at least second place in America's Vote, eliminating him from the competition in Episode 105 instead of All That! and Taylor Ware.
- J.R. Johns was the first animal act in the history of America's Got Talent to receive a "Yes" vote from a judge and also to progress to the next round of competition.
- He was the first act to receive three "X" votes in the Season 1 Semifinals.
- He was also the first act in the history of America's Got Talent to be buzzed by all of the judges in the live shows.